AI-Enhanced Single-Particle Tracking – SptPALM

Mapping Single-Molecule Dynamics

Single-Particle Tracking Photoactivated Localization Microscopy (SptPALM) is designed to map single-molecule dynamics.

• It uses a photo-activable protein called mEOS (from coral) to track proteins of interest.

• This technique has brought unprecedented insights into molecule dynamics, from neurons to plant cells.

• SptPALM inventors were awarded the Nobel Prize in 2014.

The Challenge: mEOS Blinking

One difficulty with mEOS is that it blinks, and this blinking is relative to laser power used for activation.

Biologists face a tradeoff between:

• Signal-to-noise ratio (improves with higher laser power)

• Tracking time of the protein-mEOS fusion (longer at lower laser power)

AI-Powered Solution by BionomeeX

BionomeeX, in collaboration with CNRS researchers, developed an AI-based algorithm to enhance SptPALM imaging under very low laser conditions.

• The algorithm processes videos taken at low laser power

• Achieves particle track recovery 3× longer than traditional high-laser-power imaging

• Maintains high accuracy while reducing phototoxicity and photobleaching

Try It Yourself

For more details, feel free to contact us.

We also provide free testing on your own data upon request.